Abstract This study investigates the presence of new psychoactive substances (NPS) and their metabolites in two wastewater treatment plants (WWTPs) situated in South Wales. UK (WWTP-1 and WWTP-2). Analysis was conducted for 35 NPS and metabolites. along with the inclusion of benzoylecgonine (main copyright metabolite) and cannabis. https://www.rawafricaonline.com/