Semi-quantitative RT-PCR analysis was executed to check the results from the extraction protocols over the amplification of 3 typical housekeeping genes ( : To quantify the absolute range of RNA molecules per sample, incorporate a 10‐fold dilution series of acknowledged concentrations of an in vitro−transcribed normal (see Assist Protocol). 8 https://www.facebook.com/NewTechFind/posts/pfbid0FDwXLmxi2raDNPcEpRa9jeGJbqTJ9rKxkuvCV6x8ZRC8R3wYhPw7yVXLXMNTF8JFl